FASTA FASTQ
File conversion

Convert FASTA to FASTQ

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Is it possible to convert FASTA to FASTQ?
Yes - FASTA converts to FASTQ.

FASTA -> FASTQ adds (placeholder) quality scores; only meaningful when a tool needs FASTQ input. The reverse (FASTQ -> FASTA, which the db lists) is the common, lossless direction - it just drops the quality lines. Use seqkit, seqtk, EMBOSS seqret or Biopython.

1 programs
Also to FASTQ: AB1

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Tested on macOS, Windows & Linux
Last verified Oct 2026

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How to convert FASTA to FASTQ

Converting a .FASTA file to .FASTQ lets you open the content in software that prefers the .FASTQ format, though some details may change compared with the original. The programs listed below open .FASTA files and can save or export them to .FASTQ (or a closely related format).

A full step-by-step guide for this conversion is on the way; the information above is drawn straight from our database.

Programs that convert FASTA

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