FASTA SCF
Not possible

Convert FASTA to SCF

Can you convert FASTA to SCF?
QUICK ANSWER
Is it possible to convert FASTA to SCF?
No - not a direct conversion.

The db lists scf->fasta etc. (trace formats). SCF/AB1/ABI are sequencer CHROMATOGRAM traces; you can extract a FASTA base-call FROM them, but you cannot create a real chromatogram trace FROM plain FASTA (the raw signal data doesn't exist). Convert trace -> FASTA, not the reverse.

No direct path
Also to SCF: AB1 · SEQ

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Tested on macOS, Windows & Linux
Last verified Oct 2026

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Converting FASTA to SCF

A .FASTA file and a .SCF file hold different kinds of data, so there is no direct way to turn one into the other. The programs listed below can open .FASTA files and may be able to export the content to a format closer to .SCF.

A full step-by-step guide for this conversion is on the way; the information above is drawn straight from our database.

Programs that convert FASTA

Convertrix
ChromasPro

About these formats